These cells are responsive to 5-alpha-dihydrotestosterone (growth modulation and acid phosphatase production).The cells do not produce a uniform monolayer, but grow in clusters which should be broken apart by repeated pipetting when subcultures are prepared.They attach only lightly to the substrate, do not become confluent and rapidly acidify the medium.Growth is very slow.The cells should be allowed to incubate undisturbed for the first 48 hours after subculture.When flask cultures are shipped, the majority of the cells become detached from the flask and float in the medium. Upon receipt, incubate the flask (in the usual position for monolayer cultures) for 24 to 48 hours to allow the cells to re-attach. The medium can then be removed and replaced with fresh medium.If desired, the contents of the flask can be collected, centrifuged at 300 X g for 15 minutes, resuspended in 10 mL of medium and dispensed into a single flask.
种属来源: | 人 |
---|---|
组织来源: | 前列腺 |
疾病类型: | 前列腺癌 |
细胞贴壁类型: | 贴壁 |
细胞形态: | 上皮细胞 |
细胞数: | 30-50代 |
培养基: | RPMI-1640 |
血清: | FBS |
消化液: | KGY0012 |
成瘤性: | YES |
温馨提示:因厂家更改产品包装、产地或者更换随机附件等没有任何提前通知,且每位咨询者购买情况、提问时间等不同,为此以下回复仅对提问者3天内有效,其他网友仅供参考!若由此给您带来不便请多多谅解,谢谢!
服务热线
0771-3293894