LS 174T is a variant of LS 180 that has been maintained by using trypsin in the subculture protocol. It is more easily subcultivated than that parent line and, like LS 180, it is reported to produce large amounts of carcinoembryonic antigen (CEA).Electron microscopic studies revealed abundant microvilli and intracytoplasmic mucin vacuoles. RefThey are negative for p53 antigen expression, but positive for mRNA expression.LS 174T cells stain positively for cytokeratins.The line is positive for expression of c-myc, N-myc, H-ras, N-ras, Myb, and fos oncogenes. RefK-ras and sis oncogene expression were not detected.
种属来源: | 人 |
---|---|
组织来源: | 结肠 |
疾病类型: | 结肠腺癌 |
细胞贴壁类型: | 贴壁 |
细胞形态: | 上皮细胞 |
细胞数: | 30-50代 |
培养基: | MEM |
血清: | FBS |
消化液: | KGY0012 |
成瘤性: | YES |
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