Ultrastructural features reported for HT-29 cells include microvilli, microfilaments, large vacuolated mitochondria with dark granules, smooth and rough endoplasmic reticulum with free ribosomes, lipid droplets, few primary and many secondary lysosomes. The cells express urokinase receptors, but do not have detectable plasminogen activator activity [PubMed ID: 8381394]. HT-29 cells are negative for CD4, but there is cell surface expression of galactose ceramide (a possible alternative receptor for HIV). The line is positive for expression of c-myc, K-ras, H-ras, N-ras, Myb, sis and fos oncogenes. The p53 antigen is overproduced, and there is a G -> A mutation in codon 273 of the p53 gene resulting in an Arg -> His substitution. N-myc oncogene expression was not detected.There is a G -> A mutation in codon 273 of the p53 gene resulting in an Arg -> His substitution.
种属来源: | 人 |
---|---|
组织来源: | 结肠 |
疾病类型: | 结肠癌 |
细胞贴壁类型: | 贴壁 |
细胞形态: | 上皮样 |
细胞数: | 30-50代 |
培养基: | McCoy’s 5A |
血清: | FBS |
消化液: | KGY0012 |
成瘤性: | YES |
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