简介

Studies since 1979 have shown that U-937 cells can be induced to terminal monocytic differentiation by supernatants from human mixed lymphocyte cultures,The cells are negative for immunoglobulin production and Epstein-Barr virus expression.The cells express the Fas antigen, and are sensitive to TNF and anti-Fas antibodies.In 1994, PCR and cytogenetic analyses showed that a number of stocks of U-937 were contaminated with the human myeloid leukemia cell line, K-562.In the earliest stocks available, the level of contamination was 0.6%.Distribution was discontinued in March 1994, except if required for patent purposes.A stock of CRL-1593 found to be free of K-562 was propagated continuously for 8 weeks and tested weekly by PCR.Distribution and seed stocks give DNA profiles characteristic of U-937 only.Such preparations are now offered as authentic U-937 and are believed to be free of second subpopulations.This cell line is suitable as a transfection host.

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