SuperScript® II Reverse Transcriptase (RT) is an improved version of SuperScript® RT. It is a DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, an RNA:DNA hybrid. This enzyme is genetically engineered by the introduction of point mutations in the RNase H active center to reduce RNase H activity. This structural modification eliminates degradation of RNA molecules during first-strand cDNA synthesis gives SuperScript® II RT superior performance characteristics.

• Yield – Reduced RNase H activity results in greater first-strand cDNA yields
• Size of cDNA – Generation of RT-PCR products up to 12 kb
• Thermostability – Full activity at 42°C
• Flexibility – Uses either total poly(A)+ RNA

Performance Quality Testing: SDS-PAGE purity; endodeoxyribonuclease, exodeoxyribonuclease, ribonuclease assays; yield length of cDNA product.

Unit Definition: One unit of SuperScript® II is the amount of enzyme required to incorporate 1 nmole of deoxyribonucleotide acid-precipitable material in 10 min. at 37°C using poly(A)•oligo(dT)12-18 as template•primer (3).

Unit Reaction Conditions: 50 mM Tris-HCl (pH 8.3), 40 mM KCl, 6 mM MgCl2, 1 mM DTT, 0.5 mM [3H]dTTP, 0.1 mM poly(A), 0.1 mM oligo(dT)12-18, 0.1 mg/ml BSA, enzyme in 50 µl for 10 min. at 37°C.

For Research Use Only. Not intended for any animal human therapeutic diagnostic use.

 

规格

Optimal Reaction Temperature:	42° C
Final Product Size(s):	12.3 kb less
Reverse Transcriptase:	SuperScript® II
Ribonuclease H Activity:	Reduced
Form:	Frozen
Format:	Tube(s)
Template:	ssDNA, ssRNA, RNA-DNA Hybrid
Sensitivity:	Medium
Product Size:	10,000 units
Concentration:	200 U⁄µl
Enzyme Function:	RNA dependent DNA Polymerase
Shipping Condition:	Wet Ice
Number of Reactions:	50 Reactions

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