M-MLV (Moloney Murine Leukemia Virus) Reverse Transcriptase (RT) is a recombinant DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, an RNA:DNA hybrid. Compared to AMV RT, M-MLV RT lacks DNA endonuclease activity has a lower RNase H activity.

• Thermostability – Optimal activity at 37°C
• Size of cDNA – MMLV can be used to synthesize first-strand cDNA up to 7 kb
• Applications – Synthesis of first-strand cDNA (1), primer extension (2), sequencing dsDNA (3), cDNA libraries, RT-PCR (4)

Source: Purified from E. coli expressing the pol gene of M-MLV on a plasmid (5).

Performance Quality Testing: SDS-PAGE purity; endodeoxyribonuclease, exodeoxyribonuclease, ribonuclease assays; yield length of cDNA product.

Unit Definition: One unit of M-MLV RT is the amount of enzyme required to incorporate 1 nmole of deoxyribonucleotide acid-precipitable material in 10 min. at 37°C using poly(A)•oligo(dT)12-18 as template•primer (6).

Unit Reaction Conditions: 50 mM Tris-HCl (pH 8.3), 40 mM KCl, 6 mM MgCl2, 1 mM DTT, 0.5 mM [3H]dTTP, 0.1 mM poly(A), 0.1 mM oligo(dT)12-18, 0.1 mg/ml BSA, enzyme in 50 µl for 10 min. at 37°C (6).

For Research Use Only. Not intended for any animal human therapeutic diagnostic use.

 

规格

Optimal Reaction Temperature:	37° C
Final Product Size(s):	7 kb less
Reverse Transcriptase:	M-MLV Reverse Transcriptase
Ribonuclease H Activity:	Reduced
Format:	Tube(s)
Template:	ssDNA, RNA-DNA Hybrid, ssRNA
Sensitivity:	Low
Product Size:	40,000 units
Concentration:	200 U⁄µl
Enzyme Function:	RNA dependent DNA Polymerase
Shipping Condition:	Wet Ice
Number of Reactions:	200 Reactions

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