描述

Invitrogen Tricine Gels provide separation of low molecular weight proteins and peptides. The Tricine system is a modification of the tris-glycine discontinuous buffer system developed by Schaegger and von Jagow (Schaegger and von Jagow, 1987) specifically for resolving peptides and low molecular weight proteins. In this system the tricine replaces the glycine in the running buffer, resulting in more efficient stacking and destacking of low molecular weight proteins and higher resolution of smaller peptides.

Features of Invitrogen Tricine protein gels:
• Increased resolution of proteins with molecular weights as low as 2 kDa
• Improved compatibility with direct sequencing of proteins after transferring to PVDF
• Minimized protein modification due to the lower pH of the tricine buffering system

Learn more about all of our Tricine gels >

Formulation: Invitrogen Tricine gels are made with high-purity, strictly quality-controlled reagents: Tris base, HCl, acrylamide, bisacrylamide, TEMED, APS, and highly purified water. Our Tricine gels have a 4% stacking gel and do not contain SDS. The Tricine system requires SDS in sample and running buffers for best results.

Choose the right Tricine gel for your protein separation
Invitrogen Tricine gels come in three polyacrylamide concentrations of 10%, 16%, and a gradient of 10–20%. Select from our many well formats, including 10-, 12-, and 15-well. Tricine gels are formulated for denaturing gel electrophoresis applications. For optimal sample preparation, we recommend Tricine SDS Sample Buffer and optimal separation use Tricine SDS Running Buffer.

For transfer of proteins to a membrane, we recommend using the Novex Tris-Glycine Transfer Buffer. Rapid semi-dry transfer can be performed using the Pierce Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device. Alternatively, traditional wet transfer can be performed using the XCell II Blot Module or the Mini Blot Module.

Related links
Specialized Protein Gel Support
Protein Gel 1D Electrophoresis Support

规格

For Use With (Equipment):	XCell SureLock® Mini
Gel Percentage:	10-20 %
Gel Size:	Mini (8 cm x 8 cm)
Gel Type:	Tricine Gel
Mode of Separation:	Molecular Weight
Product Line:	Novex™
Product Size:	10 gels (1 box)
Quantity:	10 gels
Sample Loading Volume:	25 µL
Separation Range:	5 kDa to 40 kDa
System:	Novex®
Thickness:	1.0 mm
Well Format:	1D Well
Wells:	10 wells
Shipping Condition:	Wet Ice

内容及储存