描述

The FilmTracer™ LIVE⁄DEAD Biofilm Viability kit provides a two-color fluorescence assay of bacterial viability, based on membrane integrity, that has proven usefule for a diverse array of bacterial genera including those growing in biofilm communities. The LIVE⁄DEAD Biofilm Viability kit utilizes mixtures of our SYTO® 9 green fluorescent nucleic acid stain and the red-fluorescent nucleic acid stain, propidium iodide. These stains differ both in their spectral characteristics and in their ability to penetrate healthy bacterial cells. When used alone, the SYTO® 9 stain generally labels all bacteria in a population—those with intact membranes and those with damaged membranes. In contrast, propidium iodide penetrates only bacteria with damaged membranes, causing a rduction in the SYTO® 9 stain fluorescence with both dyes are present. Thus, with an appropriate mixture of the SYTO™ 9 and propdium iodide stains, bacteria with intact cell membranes stain fluorescent green, whereas bacteria with damaged membranes stain fluorescent red.

规格

Color:	Green, Red
Detection Method:	Fluorescent
Emission Class:	Visible
Excitation Class:	Visible
Excitation⁄Emission (nm):	480⁄500, 635
Flow Cytometer Laser Lines:	488
For Use With (Equipment):	Confocal Microscope, Flow Cytometer, Fluorescence Microscope, Fluorescent Imager, Microplate Reader
Form:	Liquid
Label or Dye:	SYTO® 9, propidium iodide
Product Line:	FilmTracer™, Molecular Probes®
Product Size:	1 kit
Sub-Cellular Localization:	Cytoplasm & Cytosol
Shipping Condition:	Room Temperature

内容及储存