描述

The Ambion® TaqMan® Cells-to-C _T Control Kit is designed for use with the TaqMan® Gene Expression Cells-to-C _T Kit. The Control Kit includes XenoRNA™ Control, a synthetic RNA transcript with a unique sequence that lacks homology to current annotated biological sequences, making it an ideal control for any experiment. The Control Kit also includes a TaqMan® Gene Expression Assay for the XenoRNA™ Control target, and a TaqMan® Gene Expression Assay for the highly expressed endogenous control gene β-actin.

Features of the The Ambion® TaqMan® Cells-to-C_T Control Kit:
• Confirms sufficient cell input
• Monitors the presence of RT or real-time PCR inhibitors
• Controls for sample normalization

Monitor Inhibition and Confirm Sufficient Cell Input
The TaqMan® Cells-to-C _T™ Control Kit is used to assess inhibition and cell input in Cells-to-C _T reactions. For example, the XenoRNA™ Control confirms that samples can support RT-PCR and is an indicator of reaction inhibitors. By adding the XenoRNA™ Control to the Stop Solution during the last step of the cell lysis procedure, each sample is provided with a constant amount of control target and subsequently amplified by the XenoRNA™ TaqMan® Gene Expression Assay.

Amplification with the β-actin Gene Expression Assay functions as an endogenous control for normalization, and ensures that the lysis reaction contains a sufficient number of cells—this is important for experiments with fewer than ~100 cells and monitors the effectiveness of cell lysis.

Evaluate Compatibility with New Cell Lines
The TaqMan® Cells-to-C _T™ Control Kit is ideal for assessing the compatibility of untested cell lines with the TaqMan® Gene Expression Cells-to-C _T Kit to determine the maximum number of cells that can be used per lysis reaction.

规格

Control Template:	XenoRNA™ control (synthetic RNA transcript)
Format:	Tube(s)
Gene Symbol:	ACTB
Includes:	Control Primer-Probe Set(s) & Template(s)
Internal Probe Modification:	FAM (5'), MGB (Minor Groove Binder) (3'), Non-Fluorescent Quencher (3')
Label or Dye:	FAM™
Product Line:	Ambion™, Applied Biosystems™, Cells-to-CT™, TaqMan™
Product Size:	100 reactions
Target Gene:	β-actin, XenoRNA™ control
Green Features:	Fewer resources used, Less hazardous, Less waste
Shipping Condition:	Dry Ice

内容及储存