描述
Phalloidin is a peptide toxin isolated from the Amanita phalloides mushroom. Phalloidin selectively binds F-actin and functions to stablilize actin polymers by inhibiting the dissociation of actin monomers from the filament ends. Because F-actin polymers compose the cytoskeleton of almost all eukaryotic cells, phalloidin conjugates can be useful probes for visualizing the structure of live or fixed cells using microscopy. Phalloidin eFluor™ 570 can be used to label cells of multiple species.
Phalloidin eFluor™ 570 is supplied as a frozen liquid. To prepare the 1000X stock solution, thaw the vial, add 30 uL high-quality anhydrous DMSO, mix and briefly spin down. The resulting solution (phalloidin DMSO stock) should be aliquoted, protected from light and moisture, and stored at -20°C. To prepare a 1X working solution of phalloidin conjugate, add 1 uL of 1000X phalloidin DMSO stock solution to 1mL of PBS containing 1% BSA.
Emit
570 nm
Excite
555 nm
Reported Application
Immunocytochemistry, Immunohistochemical Staining, Microscopy
规格
Label or Dye: | eFluor 570 |
Sub-Cellular Localization: | Cytoskeleton |
Excitation⁄Emission (nm): | 555/570 |
Product Line: | eBioscience™ |
Product Size: | 300 tests |