描述

The BacLight™ Bacterial Membrane Potential Kit provides the fluorescent membrane-potential indicator dye, DiOC ₂(3), along with a proton ionophore (CCCP) and premixed buffer. DiOC ₂(3) at low concentrations exhibits green fluorescence in all bacterial cells, but it becomes more concentrated in healthy cells that are maintaining a membrane potential, causing the dye to self-associate and the fluorescence emission to shift to red. The red- and green-fluorescent bacterial populations are easily distinguished using a flow cytometer. CCCP is included in the kit for use as a control because it eradicates the proton gradient, eliminating bacterial membrane potential.

View additional information about all microbiology assays for flow cytometry.

规格

Detection Method:	Fluorescent
Excitation⁄Emission (nm):	482⁄497
Flow Cytometer Laser Lines:	488
For Use With (Equipment):	Flow Cytometer
Indicator Properties:	Fluorescent Dye-Based
Indicator Type:	Membrane Potential Probes
Label or Dye:	DiOC2(3)
Number of Reactions:	100
Product Line:	BacLight™
Product Size:	1 kit
Shipping Condition:	Wet Ice

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