描述
AccuPrime™ SuperMix II is a ready-to-use mixture of DNA polymerase, accesory proteins, salts, magnesium, and dNTPs for efficient PCR amplification. All you have to do is add template and primers, reducing set-up time by half (Figure 1).
For accurate PCR performance, AccuPrime™ SuperMix II sets the standard. You'll amplify your DNA targets cycle after cycle, eliminating troubleshooting and rework. In addition to a powerful DNA polymerase, AccuPrime™ SuperMix I includes Platinum® anti-DNA polymerase antibodies that inhibit polymerase activity, providing an automatic hot-start, and a thermal stable accessory protein that enhances specific primer-template hybridization during every cycle of PCR (Figure 1). This unique combination allows for success, even under previously suboptimal conditions.
AccuPrime™ SuperMix II is designed for amplification of genomic DNA amplicons, plasmid DNA, or cDNA templates up to 4kb in length. Alternatively, AccuPrime™ SuperMix I is optimized to amplify a tighter range of targets less than 200bp in length. 规格
Polymerase: | AccuPrime™ Taq DNA Polymerase |
Hot Start: | Built-In Hot Start |
Fidelity (vs. Taq): | 2 X |
GC-Rich PCR Performance: | Low |
Reaction Format: | SuperMix or Master Mix |
Product Overhang: | 3'-A |
Reaction Speed: | Standard |
Final Product Size(s): | 4 kb or less |
Exonuclease Activity: | 5' - 3' |
Product Line: | AccuPrime™ |
Product Size: | 200 reactions |
Shipping Condition: | Dry Ice |
内容及储存
AccuPrime™ SuperMix II is supplied at 2X concentration to allow approximately 50% of the final reaction volume to be used for the addition of primer and template solutions. Reagents sufficient for 200 amplification reactions of 25 µl each are provided.
AccuPrime™ SuperMix II Components:
40 mM Tris-HCl (pH 8.4); 100 mM KCl; 3 mM MgCl2, 400 µM each dGTP, dATP, dTTP, dCTP; AccuPrime™ Taq DNA Polymerase; thermostable AccuPrime™ protein; stabilizers.
200 rxns: 2 × 1.25mL
Store at -20°ree;C in a non-frost-free freezer.