描述
Premo™ Cellular Hydrogen Peroxide Sensor is a genetically encoded sensor that is transduced/transfected into cells, enabling dynamic measurement of hydrogen peroxide (H
2O
2) in live cells. This detection system responds to stimuli quickly and is reversible. With the incorporated BacMam technology, this sensor is ready to use—simply add to cells in growth media and incubate overnight.
The roGFP-Orp1 Chimera Premo™ Cellular Hydrogen Peroxide Sensor combines the selectivity of a roGFP-Orp1 chimera with the transduction efficiency of BacMam 2.0 technology. Redox-senstive green fluorescent protein (roGFP) detects redox potential through two cysteines introduced into the β-barrel structure of the GFP protein. Dilsulfide formation between the cysteines leads to protonation of GFP and increases the 400 nm excitation spectra at the expense of the 488 nm excitation spectra. The fluorescence at the two different excitation wavelengths can be quantitated using the same emission wavelength of 515 nm. Orp1 is a hydrogen peroxide-sensitive yeast protein that forms disulfides upon reaction with hydrogen peroxide and has the ability to transduce signals to other redox proteins by disulfide exchange. By combining the hydrogen peroxide-selective properties of Orp1 and roGFP, it is possible to selectively quantitate hydrogen peroxide in cells.
BacMam Technology The incorporated BacMam technology is based on the use of an insect cell virus (baculovirus) to efficiently deliver and express genes in mammalian cells. Because mammalian cells do not support replication of baculoviruses, transduction is extremely well tolerated and generally lacking in cytopathic effects, even at high virus levels.
Learn more about BacMam technology.
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