描述

The InVision™ His-tag In-gel Staining Kit includes InVision™ His-tag In-gel Stain (also available separately), as well as BenchMark™ His-tagged Protein Standard. InVision™ His-tag In-gel Stain is a ready-to-use, fast, sensitive, and highly specific stain for visualizing His-tagged fusion protein bands directly in a polyacrylamide gel following electrophoresis. Using InVision™ His-tag In-gel Stain you will:

• Stain proteins fused to an oligohistidine sequence
• Eliminate western blotting-detect His-tagged proteins directly in the gel
• Detect nanogram levels of protein bands with a UV transilluminator, equipped with a standard camera, or a laser-based scanner
• Visualize results in under 3 hours

Convenienty provided as one ready-to-use solution, InVision™ His-tag In-gel Stain does not require mixing, dilution, or solution preparation.

Fast and Easy Staining
The InVision™ His-tag In-gel Stain protocol is easy to perform and requires very little hands-on-time, allowing for rapid and scalable protein expression screening in a variety of gel types. Following electrophoresis, the gel is fixed, incubated in the stain, washed, and the specific His-tagged fusion protein detected by exposing the gel to UV or visible light. In under 3 hours, nanogram levels of His-tagged fusion protein can be detected (Figure 1). After documenting your InVision™ Stain signal, total protein staining can be performed.

规格

Target Molecule:	Proteins (His-Tagged)
Label or Dye:	InVision™ His-Tag Stain
Detection Method:	Fluorescent
Detection Location:	In-Gel Detection
Product Line:	InVision™
Product Size:	1 kit

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