描述
ProBond™ Nickel-Chelating Resin is a nickel-charged affinity resin used to purify recombinant proteins containing a polyhistidine (6xHis) sequence. Proteins bound to the resin may be eluted with either low pH buffer or by competition with imidazole or histidine. One-step purification can be performed under both native and denaturing conditions. ProBond™ Resin uses the chelating ligand iminodiacetic acid (IDA) coupled to a highly cross-linked 6% agarose resin that is suitable for use in FPLC, batch, and gravity flow applications. 规格
| Column Type: | Affinity Column |
| Format: | Suspension |
| Immobilized Phase: | Nickel-Chelating |
| Product Line: | ProBond™ |
| Product Size: | 150mL |
内容及储存
The ProBond™ resin is pre-charged and able to bind 1-5 mg of recombinant protein per 1mL of resin. It is provided as a 50% slurry in 20% ethanol. The resin will appear blue in color when charged with Ni 2+ . Store at +4°C. ProBond™ resin is guaranteed stable for 6 months when properly stored.
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