描述
ProBond™ 镍螯合树脂是用于纯化含有多组氨酸 (6xHis) 序列的重组蛋白质的含镍亲和树脂。 用低 pH 缓冲液或者通过咪唑或组氨酸的竞争洗脱树脂上结合的蛋白质。 可在天然和变性条件下进行一步法纯化。 ProBond™ 树脂使用与高度交联的 6% 琼脂糖树脂偶联的螯合配体亚氨基二乙酸 (IDA),琼脂糖树脂适用于 FPLC、批量和重力流应用。 规格
Column Type: | Affinity Column |
Format: | Suspension |
Immobilized Phase: | Nickel-Chelating |
Product Line: | ProBond™ |
Product Size: | 50mL |
内容及储存
The ProBond™ resin is pre-charged and able to bind 1-5 mg of recombinant protein per 1mL of resin. It is provided as a 50% slurry in 20% ethanol. The resin will appear blue in color when charged with Ni 2+ . Store at +4°C. ProBond™ resin is guaranteed stable for 6 months when properly stored.