描述

Invitrogen NuPAGE Tris-Acetate protein gels provide excellent separation of large molecular weight proteins. NuPAGE Tris-Acetate protein gels are high performance polyacrylamide gels that simulate the denaturing or the native conditions of the traditional laemmli system. Its unique buffer formulation maintains a low operating pH during electrophoresis, resulting in superior resolution of proteins compared to traditional Tris-glycine SDS-PAGE gels.

Features of NuPAGE Tris-Acetate gels:
• Separate a wide range of molecular weight proteins
• Superior protein band resolution and stability
• Preserve protein sample integrity using optimized sample preparation processes

Learn more about all of our NuPAGE Tris-Acetate gels >

Choose the right NuPAGE Tris-Acetate gel for your protein separation
Obtain optimal separation of your proteins by choosing the right combination of gel and running buffer. NuPAGE Tris-Acetate protein gels come in a polyacrylamide concentration of 7% and a 3-8% gradient. Gels come in two sizes: mini (8 cm x 8 cm) or midi (8.7 cm x 13.3 cm) and either 1.0 mm (mini and midi gels) or 1.5 mm (mini gel format only) in thickness. NuPAGE Tris-Acetate gels also come in multiple well formats.

Run your proteins in native or denatured form
NuPAGE Tris-Acetate protein gels do not contain SDS and so can be used to separate proteins in native or denatured form. For denatured proteins, we recommend using NuPAGE LDS Sample Buffer and NuPAGE Tris-Acetate SDS Running Buffer. For native proteins, we recommend using Novex Tris-Glycine Native Sample Buffer and Novex Tris-Glycine Native Running Buffer. The gels can be run using our XCell SureLock Mini-Cell or the Mini Gel Tank.

For transfer of proteins to a membrane, we recommend using NuPAGE Transfer buffer. Rapid semi-dry transfer can be done using the Pierce Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device. Alternatively, traditional wet transfer can be performed using the XCell II Blot Module or the Mini Blot Module.

Related links
Overview of 1D Protein Electrophoresis

规格

For Use With (Equipment):	XCell SureLock® Mini
Gel Percentage:	3-8 %
Gel Size:	Mini (8 cm x 8 cm)
Gel Type:	Tris-Acetate Gel
Mode of Separation:	Molecular Weight
Product Line:	Novex™, NuPAGE®
Product Size:	10 gels (1 box)
Quantity:	10 gels
Sample Loading Volume:	15 µL
Separation Range:	40 kDa to 500 kDa (TA Buffer)
System:	NuPAGE® Novex®
Thickness:	1.0 mm
Well Format:	1D Well
Wells:	15 wells
Shipping Condition:	Wet Ice

内容及储存