描述
PureLink™ Quick Gel Extraction & PCR Purification Combo Kit offers the ability to perform both a gel extraction or a PCR purification a single kit.
The PureLink™ Quick Gel Extraction and PCR Purification Combo Kit is designed to purify DNA fragments from agarose gels. The simple procedure uses a silica-based spin cartridge to purify DNA fragments from 40 bp to 10 kb in < 30 minutes. It also provide all components to perform a rapid and efficient removal of short primers, dNTPs, enzymes, short-failed PCR products, and salts from PCR fagments >100 bp in less than 10 minutes. Isolated DNA using the PureLink™ kit is free of proteins, dye, and agarose, and is ready-to-use for a variety of applications, including automated fluorescent DNA sequencing, manual DNA sequencing, PCR, in vitro transcription, restriction mapping, cloning, and labeling. The kit contains all the buffers from the PureLink™ Quick Gel extraction kit and the PCR binding buffer from PureLink™ PCR Purification kit with Invitrogen branded lidded column. 规格
DNA Fragment Size: | Gel: purifies 40 bp to 10 kb, PCR: purifies >100 bp |
Downstream Application: | Cloning, In Vitro Transcription, Next-Generation Sequencing, Nucleic Acid Labeling, PCR, Sequencing |
Elution Volume - max: | 100 µl |
Elution Volume - min: | 30 µl |
Final Product: | DNA |
High Throughput Compatibility: | Not High Throughput-Compatible (Manual) |
In-Gel Dye: | Ethidium Bromide, SYBR® Safe |
Isolation Technology: | Silica Spin Column |
Product Line: | PureLink™ |
Product Size: | 50 preps |
Purification Time: | 20 min |
Purity or Quality Grade: | Molecular Biology Grade |
Quantity: | 1 kit |
Sample Type (General): | Gel Samples |
Sample Type (Specific): | DNA Fragments in Agarose Gel Slices, PCR Products |
Scale: | Mini |
System: | PureLink™ |
Yield (up to): | 40 µg |
Shipping Condition: | Room Temperature |
内容及储存
The PureLink™ Quick Gel Extraction & PCR Purification Combo Kit includes sufficient PureLink™ columns, collection tubes, elution tubes, and reagents for 50 preps. Store components at room temperature. All reagents are guaranteed stable for 6 months when properly stored