描述
Invitrogen™ ezDNase™ Enzyme is a recombinant double-strand-specific DNase for the fast removal of contaminating genomic DNA from RNA preparations. It cleaves phosphodiester bonds in double-stranded DNA to yield 2–8 bp oligonucleotides with 5’-phosphate and 3’-hydroxyl termini.
Features of ezDNase Enzyme include:
• Efficient and fast genomic DNA removal
• Highly specific—no impact on RNA, cDNA, or primers in RT reactions
ezDNase Enzyme's high specificity for double-stranded DNA enables efficient and fast genomic DNA removal without reduction in the quality or quantity of RNA or single-stranded DNA present in the reaction such as cDNA and primers. ezDNase Enzyme is heat-labile and so can be easily deactivated by heat treatment at moderate temperature (55°C). These features make ezDNase a superior choice for genomic DNA removal prior to reverse transcription reactions.
When used in combination with the
SuperScript™ IV VILO™ Master Mix or other Invitrogen reverse transcription reagents, ezDNase Enzyme helps significantly reduce the possibility of cDNA synthesis being compromised by genomic DNA and reduce variation in RT-qPCR quantitation caused by RNA loss or damage during conventional DNase treatment.
内容及储存
• ezDNase Enzyme, 50 µL
• ezDNase Buffer (10X), 100 µL
• Nuclease-free water, 1.25mL
Store at -5 to -30°C.
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