描述
Like CFDA SE, CellTrace™ Oregon Green® 488 (carboxy-DFFDA SE) should be a useful tool for following proliferating cells. This Oregon Green® 488 probe passively diffuses into cells, where it is colorless and nonfluorescent until its acetate groups are removed by intracellular esterases to yield a highly fluorescent, amine-reactive dye. Upon reaction with intracellular amines, the probe forms Oregon Green® 488 conjugates that are well-retained by cells. Unlike fluorescein conjugates, Oregon Green® 488 conjugates exhibit bright, photostable green fluorescence that is independent of pH at typical cellular pH values (pH-6-8). 规格
Reagent Type: | Cell Labeling Reagents, Cell Tracker Compounds |
Detection Method: | Fluorescent |
Excitation⁄Emission (nm): | 496⁄524, 497⁄592 |
Flow Cytometer Laser Lines: | 488 |
For Use With (Equipment): | Flow Cytometer, Fluorescence Microscope |
Form: | Solid |
Label or Dye: | Oregon Green® 488 |
Product Line: | CellTrace™, Oregon Green® |
Product Size: | 20 x 50 µg |
Solubility: | DMSO (Dimethylsulfoxide) |
Shipping Condition: | Room Temperature |
内容及储存
Contains 20 vials of Oregon Green® 488 carboxylic acid diacetate, SE (50 µg/vial).
Store in freezer (-5 to -30°C) and protect from light.