描述
ProcartaPlex™ immunoassays are our ideal solution for assessing multiple protein biomarkers in a single sample. Our ProcartaPlex assays use Luminex ™ xMAP technology for the multi-analyte detection of secreted proteins including cytokines, chemokines, growth factors and other proteins.
ProcartaPlex™ assays are subject to the same stringent and robust development and manufacturing as are our extensively validated Platinum and Instant ELISA kits. Quantitate multiple targets in cell/tissue culture supernatants, serum, and plasma.
Explore our broad portfolio of pre-configurated panels and combinable single analytes.
This kit does not contain a Multiscreen Filter Plate.
Reactivity/Species
Human
Suitable Sample Types
cell culture supernatant, serum, plasma
Sample Volume
Serum, Plasma: 25 µL; CCS: 50 µL
Reported Application
Multiplex Immunoassay
Target List
MMP-1; MMP-7; MMP-8; MMP-12; MMP-13
Bead Regions
MMP-1: 64; MMP-7: 65; MMP-8: 67; MMP-12: 47; MMP-13: 51
Assay Range
[As determined for Lot 1:] MMP-1: 7.84-32100 pg/mL; MMP-7: 7.37-30200 pg/mL; MMP-8: 25.17-103100 pg/mL; MMP-12: 4.30-17600 pg/mL; MMP13: 5.76-23600 pg/mL
Assay Sensitivity
[As determined for Lot 1:] MMP-1: 0.04 pg/mL; MMP-7: 0.04 pg/mL; MMP-8: 8.0 pg/mL; MMP-12: 1.4 pg/mL; MMP-13: 2.1 pg/mL
规格
Former Identifiers: | eBioscience |
Detection Method: | Fluorescent |
For Use With (Equipment): | Luminex™ Instruments |
Immunoassay Kit Format: | Panel |
Interassay CV: | Less than 10% |
Intraassay CV: | Less than 10% |
Label or Dye: | R-PE |
Multiplex Panel Name: | MMP |
Multiplex Panel Version: | 1 |
Multiplex Size (Plex No.): | 5 |
Product Line: | ProcartaPlex™ |
Product Size: | 96 tests |
Sample Type (Specific): | Cell Culture Supernatants, Plasma, Serum |
Sample Volume: | CCS: 50 µL, Serum, Plasma: 25 µL |
System: | Luminex™ |
Target Species (Named): | Human |
Target Species (Validated): | Human |
内容及储存
2 vials Human MMP Standard Mix
1 vial Human MMP Bead Mix
1 vial Human MMP Detection Antibody Mix
1 bottle Reading Buffer
1 bottle Wash Buffer 10x
1 bottle Streptavidin-PE
1 bottle Universal Assay Buffer
1 bottle Detection Antibody Diluent
PCR 8-Tube Strip
Adhesive Film
Greiner Microplate black 96 well
Store at 2-8°C.