描述
Gateway® Technology enables rapid cloning of one or more genes into virtually any protein expression system. Once you have an entry clone, you can recombine your gene of interest into a variety of expression vectors adapted for use in Gateway® Technology. The PCR Cloning System with Gateway® Technology is designed to create an entry clone following PCR with attB-modified custom primers (Figure 1). The PCR product is directionally cloned with efficiencies typically >99%. The PCR Cloning System with Gateway® Technology offers:
• Rapid, efficient, directional cloning of PCR products
• Versatility to use any amplification enzyme
• Cloning without the need for restriction endonucleases or ligase
• Selection of entry clones with kanamycin or Zeocin™
In addition, the pDONR™221 and pDONR™/Zeo vectors have a pUC origin for high plasmid yields and universal M13 sequencing sites for ease of use. 规格
| Vector Type: | pDONR |
| Cloning Method: | Gateway® |
| Product Line: | Gateway®, OmniMAX™, pDONR™ |
| Product Size: | 20 reactions |
内容及储存
The PCR Cloning Kit with Gateway® Technology includes Gateway® BP Clonase® II enzyme mix and reaction buffer, Gateway® pDONR™221 or pDONR™/Zeo vector, M13 sequencing primers, PEG/Mg 2+ solution, proteinase K, pEXP7-tet positive control, and One Shot® OmniMAX™2 Competent Cells. Store competent cells and enzyme mix at -80°C. Store all other components at -20°C.
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