描述
Lumio™ Technology is designed for simple fluorescent detection of recombinant protein. The Lumio™ tag is a small (six amino acid) sequence that binds a fluorescent substrate, allowing you to visually detect protein localization in mammalian cells. The Mammalian Lumio™ Gateway® vectors (Figure 1) offer the following features:
• Lumio™ tag for accurate in vitro and in vivo protein detection
• CMV promoter for high-level constitutive expression
• tR sites for efficient recombination with Gateway® entry clones
• Blasticidin resistance gene for fast, efficient selection
Use Mammalian Lumio™ Gateway® vectors for reliable and consistent protein expression, detection, and localization in mammalian cells (Figure 2). Vectors are available for generating protein with an N- and C-terminal Lumio™ fusion tag. In addition, each Mammalian Lumio™ Gateway® Kit includes a Lumio™ Green, Red, or Dual In-Cell Detection Kit. The Dual In-Cell Detection Kit is ideal for doing two color "pulse-chase" experiments to examine protein localization over time. 规格
| Color: | Green |
| Detection Location: | In-Cell Detection |
| Detection Method: | Fluorescent |
| For Use With (Equipment): | Electron Microscope, Fluorescence Microscope |
| Label or Dye: | Lumio™ Green |
| Product Line: | Lumio™ |
| Product Size: | 1 kit |
内容及储存
Mammalian Lumio™ Gateway® Vectors include 6 µg pcDNA™6.2/nLumio™-DEST, 6 µg pcDNA™ 6.2/cLumio™-DEST™, 10 µg pcDNA™6.2/nLumio™-GW/p64 control vector, and the indicated Lumio™ In-Cell Detection Kit. The Lumio™ In-Cell Detection Kits include 40 µl of Lumio™ Green or Red (Dual contains both Green and Red) fluorescent reagent as a 2 mM stock solution in DMSO. Store all reagents at -20°C. Guaranteed stable for 6 months when properly stored.
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