描述

This kit is designed for use with the Power SYBR® Green (SKU#s 4402953, 4402954, and 4402955) and Fast SYBR® Green Cells-to-C _T™ Kits (SKU#s #4402956 and 4402957). Sufficient reagents are provided for 100 reactions. It includes Xeno™ RNA Control, a synthetic RNA transcript with a unique sequence that lacks homology to current annotated biological sequences, and a PCR primer set for the Xeno™ RNA Control target. It also includes a PCR primer set for the highly expressed endogenous control gene β-actin (ACTB). Together these reagents provide positive controls for reverse transcription and SYBR® Green-based real-time PCR, and can indicate the presence of any reverse transcription or PCR inhibitors. Real-time RT-PCR with SYBR® ACTB Primers can also provide an endogenous control for sample normalization, as well as to confirm sufficient cell input in experiments with samples consisting of more than 100 cells per lysis.

规格

3' Primer Modification:	None
5' Primer Modification:	None
Control Template:	XenoRNA™ control (synthetic RNA transcript)
Format:	Tube(s)
Gene Symbol:	ACTB
Includes:	Control Primers & Template(s)
Label or Dye:	Unconjugated
Product Line:	Applied Biosystems™, Cells-to-CT™, SYBR®
Product Size:	100 reactions
Target Gene:	β-actin, XenoRNA™ control
Green Features:	Fewer resources used, Less hazardous, Less waste
Shipping Condition:	Dry Ice

内容及储存