描述
Invitrogen's™ pLenti6.3⁄V5™-TOPO®Cloning Kit is part of our ViraPower™ HiPerform™ Lentiviral Expression System (catalog K5310-00).
The pLenti6.3⁄V5™-TOPO® -TA Cloning Kit contains the TOPO®- adapted ViraPower™ HiPerform™ lentiviral expression vector, pLenti6.3⁄V5™-TOPO® for quick PCR-based cloning and high-level expression of a target gene in dividing and non-dividing mammalian cells. The pLenti6.3⁄V5™-TOPO® vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central Polypurine Tract (cPPT) sequence from the HIV-1 integrase gene to produce at least 4-fold increase in protein expression compared to vectors lacking these elements.
Advantages
• Stable expression
• Long-term experiments
• Accurate titer of functional virus
• 5 minute, high-efficiency TOPO® PCR cloning in three simple steps
Key Features
• HiPerform™ WPRE and cPPT elements
• CMV promoter
• V5 epitope tag at C terminus
• Blasticidin selection
Kit includes
• A lacZ vector as a positive control, pLenti6.3⁄V5-GW⁄lacZ™
• Stbl3™ competent cells
For research use only. Not intended for any therapeutic or diagnostic use. 规格
| Promoter: | CMV |
| Key Function: | Viral Expression |
| Protein Tag or Fusion: | V5 Epitope Tag |
| Delivery Method: | Lentiviral |
| Cloning Method: | TOPO®-TA |
| Selection Agent (Eukaryotic): | Blasticidin |
| Vector Type: | TOPO-TA Vectors, pLenti |
| Constitutive or Inducible System: | Constitutive |
| Product Line: | HiPerform™, TOPO®, ViraPower™ |
| Product Size: | 1 kit |
| Selection Marker Promoter: | SV40 Promoter |
内容及储存
TOPO® TA Cloning Reagents (Box 1)
pLenti6.3?V5™-TOPO® (5-10 ng⁄µL): 20 µL, -20°C
pLenti6.3⁄V5-GW⁄lacZ™ Control vector (0.5 µg⁄µL): 20 µL, -20°C
10X PCR Buffer: 100 µL, -20°C
dNTP Mix: 10 µL, -20°C
Salt Solution: 50 µL, -20°C
CMV Forward Primer (100 ng⁄µL): 20 µL, -20°C
V5 (C-term) Reverse Primer (100 ng⁄µL): 20 µL, -20°C
Control PCR Template (50 ng⁄µL): 10 µL, -20°C
Control PCR Primers (100 ng⁄µL): 10 µL, each -20°C
Sterile Water: 1mL
One Shot® Stbl3™ Chemically Competent E. coli (Box 2)
Stbl3™ Cells: 21 x 50 µL, -80°C
pUC19 control DNA (10 pg⁄µL): 50 µL, -80°C
S.O.C. Medium: 6mL, -80°C
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