描述

The Gal-Screen® assay system combines direct cell lysis with rapid, ultra-sensitive chemiluminescent detection of β-galactosidase reporter enzyme.

• Homogeneous assay format allows detection of β-galactosidase in the presence of normal culture media without removal of media and without an additional cell lysis step, providing the easiest and most streamlined assay procedure possible.
• The kinetics of the glow-assay provides a "window" during which measurements may be performed, facilitating HTS applications where assay automation is used.
• Can be used with either mammalian or yeast model systems, providing flexibility in choice of model systems.
• Wide dynamic range of β-galactosidase assay enables accurate measurement of enzyme from femtogram to nanogram range.
• Assay sensitivity is 100 to 1,000-fold better than either the isotopic⁄non-isotopic assays for chloramphenicol acetyl transferase (CAT) or the colorimetric⁄fluorescent assays for β-galactosidase, providing greater sensitivity than competing assay technologies.
• Highly sensitive assay with a wide dynamic range permits detection of high and low levels of reporter without performing numerous sample dilutions.
• Non-radioactive reporter gene assay kit eliminates concerns over use of radioisotopes.
• Assay can be completed in about one hour, providing fast assay turnaround.

Ideal for Screening
This homogeneous assay is ideally suited for screening applications where assay automation is required. The Gal-Screen® system uses Galacton-Star® chemiluminescent substrate for convenient measurement in a luminometer. Light emission reaches maximum in 60-90 minutes and remains constant for 45-90 minutes.

For Research Use Only. Not for use in diagnostics procedures.

规格

Assay Type:	β-Gal (lacZ) Assay
Compatible Cells:	Mammalian Cells, Yeast Cells
Detection Method:	Chemiluminescent
For Use With (Equipment):	Luminometer (Microplate)
Form:	Liquid
Format:	96-well plate
High Throughput Compatibility:	High Throughput-Compatible
Product Line:	Applied Biosystems™, Gal-Screen™, NovaBright™
Product Size:	1,000 assays
Readout:	End Point
Sample Type (Specific):	Cell Cultures
Substrate:	Galacton-Star®
Substrate Properties:	Chemical Substrate
Substrate Type:	Beta-Gal Substrate
Target Enzyme:	Beta-Galactosidase
Technique:	Enhanced Chemiluminescence

内容及储存