描述

MegaX DH10B™ T1 ^R Electrocomp™ Cells are the highest-efficiency electrocompetent cells available. Improved production processes now guarantee a three-fold greater number of colonies per transformation (>3 x 10 ¹⁰ cfu/µg of pUC DNA), making the MegaX DH10B™ T1 ^R cells ideal for highly demanding cloning and library construction. They have the same genotype as the widely used DH10B™ T1 ^R strain, including tonA, which prevents infection by T1 and T5 lytic bacteriophages, and safeguards your valuable clones and libraries.

Using MegaX DH10B™ T1^R cells
MegaX DH10B™ T1 ^R cells provide highly efficient transformation of methylated DNA, since they lack the E. coli K12 restriction systems ( mcrA Δ( mrr- hsdRMS- mcrBC)). The Φ80 lacZΔM15 marker provides α-complementation of the β-galactosidase gene, allowing blue/white screening on agar plates containing X-gal or Bluo-gal.

Genotype: F- mcrA Δ( mrr- hsdRMS- mcrBC), Φ80 lacZΔM15 Δ lacX74 recA1 endA1 araD139 Δ( ara, leu)7697 galU galK λ ^- rpsL nupG tonA

规格

Competent Cell Type:	Electrocompetent
Species:	E. coli
Format:	Tube(s)
Transformation Efficiency:	> 3x10^10
Bacterial or Yeast Strain:	DH10B™
Product Line:	DH10B™, Electrocomp™
Blue-White Screening:	Yes
Cloning Methylated DNA:	Yes
Cloning Unstable DNA:	Not Suitable for cloning unstable DNA
F' Episome (to make ssDNA):	Lacks F' Episome
High Throughput Compatibility:	Not High Throughput-Compatible (Manual)
Improves Plasmid Quality:	Yes
Plasmid Type:	May be used for plasmids > 100 kb
Preparing Unmethylated DNA:	Not Suitable for preparing unmethylated DNA
Product Size:	25 transformations
Propagating ccdB Vectors:	Not for ccdB vector propagation
Reduces Recombination:	Yes
T1 Phage - Resistant (tonA):	Yes
Shipping Condition:	Dry Ice

内容及储存