描述
The CellsDirect™ One-Step qRT-PCR Kit delivers highly sensitive and specific real-time qPCR results directly from cells, without the need for an RNA purification step.
Features of the CellsDirect™ One-Step qRT-PCR Kit include: •
Efficiency—Offers significant time and cost savings for increased throughput
•
Convenience—Enzyme mix includes both SuperScript® III Reverse Transcriptase and Platinum®
Taq DNA Polymerase
•
Sensitivity—No separate RNA isolation step increases sensitivity by minimizing sample loss
•
Broad Detection Range—Detect targets from samples ranging from a single cell to 10,000 cells
•
Versatility—Compatible with a wide variety of cell lines and samples collected by Laser Capture Microdissection (LCM)
Streamline Your Workflow In traditional real-time quantitative RT-PCR (qRT-PCR), you first isolate RNA from cells in a time-consuming procedure that can lead to loss of material. Using the CellsDirect™ One-Step qRT-PCR Kit, you lyse the cells and add the complete lysate directly to a one-step qRT-PCR reaction with minimal handling and sample loss.
Ideal for Your qRT-PCR Application CellsDirect™ technology is validated for gene expression studies involving limited samples, and is suitable for validation of RNAi knockdowns. CellsDirect™ One-Step qRT-PCR Kits are especially valuable for high-throughput applications by eliminating costly, time-consuming RNA purification steps (Figure 1). The CellsDirect™ One-Step qRT-PCR Kit may also be used to detect genomic DNA targets.
CellsDirect™ One-Step qRT-PCR Kits may be used in conjunction with hydrolysis probes (e.g. TaqMan®) or LUX™ fluorogenic primers.
Note: This kit includes 2X Reaction Mix containing ROX™ Reference Dye. An equivalent kit (
11753-500), containing a separate tube of ROX™ Reference Dye, is also available.
内容及储存
• 10mL Resuspension Buffer
• 1mL Lysis Enhancer
• 2 × 1.25mL DNase I, Amplification Grade (1 U/µl)
• 800 µl 10X DNase I Buffer
• 2 × 1mL 25 mM EDTA
• 500 µl SuperScript® III RT/Platinum® Taq Mix
• 12.5mL 2X Reaction Mix with ROX™
• 1mL 50 mM MgSO 4
• 12.5mL DEPC-treated water
• 10 µl HeLa Total RNA (10 ng/µl)
The kit is shipped on dry ice and should be stored at -20°C
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