描述

Premo™ Cellular Redox Sensor is a genetically encoded sensor that is transduced/transfected into cells, enabling dynamic measurement of glutathione redox potential or oxidative stress in live cells. This detection system responds to stimuli quickly and is reversible, and can be readily combined with other dyes for multiplexed measurement of other relevant markers. Premo™ Cellular Redox Sensor can be used as an alternative method of detecting redox potential or oxidative stress, in concert with organic-based fluorescent dyes such as CellROX®, 2',7'-dichlorodihydrofluorescein diacetate (H2-DCFDA), or ThiolTrackers™ dyes. With the incorporated BacMam technology, this sensor is ready to use—simply add to cells in growth media and incubate overnight.

The roGFP-Grx1 Chimera
Premo™ Cellular Redox Sensor combines the selectivity of a roGFP-Grx1 chimera with the transduction efficiency of BacMam 2.0 technology. Redox-senstive green fluorescent protein (roGFP) detects reactive oxygen through two cysteines introduced into the β-barrel structure of the GFP protein. Dilsulfide formation between the cysteines leads to protonation of GFP and increases the 400 nm excitation spectra at the expense of the 488 nm excitation spectra. The fluorescence at the two different excitation wavelengths can be quantitated using the same emission wavelength of 515 nm. roGFP responds to levels of GSH/GSSG through electron exchange with glutaredoxin (GRX1). The availability of GRX1 to this dynamic reaction is ensured through the linking of GRX1 to roGFP, enabling roGFP to respond faster to stimulus and be more sensitive to GSH/GSSG with reversible functionality.

BacMam Technology
The incorporated BacMam technology is based on the use of an insect cell virus (baculovirus) to efficiently deliver and express genes in mammalian cells. Because mammalian cells do not support replication of baculoviruses, transduction is extremely well tolerated and generally lacking in cytopathic effects, even at high virus levels. Learn more about BacMam technology.

规格

Indicator Type:	Free Radical Sensor
Indicator Properties:	Fluorescent Protein-Based
Excitation⁄Emission (nm):	400, 488⁄515
Product Line:	Premo™
Product Size:	5mL

内容及储存