描述
The TrackIt™ Loading Buffers are a 6X solution used for easy loading and tracking of DNA samples in agarose gels. These loading buffers contain two tracking dyes, one that runs behind the sample and one that runs ahead of the sample. The tracking dyes serve as visual markers for following migration during electrophoresis and indicate when maximum resolution of the DNA fragments has been achieved. Additionally, visualization of DNA bands will not be obscured by the tracking dyes because they run outside the limits of most DNA samples.
TrackIt™ Cyan/Orange Loading Buffer is recommended for DNA fragments between 10 bp and 1 Kb.
TrackIt™ Cyan/Yellow Loading Buffer is recommended for DNA fragments between 100 bp and 10 Kb. 规格
Buffer Type: | Sample Loading Buffers |
Concentrated: | 6 X |
Gel Compatibility: | Agarose Gels, E-Gel® |
Label or Dye: | Orange G, Xylene Cyanol |
Product Line: | TrackIt™ |
Product Size: | 3 x 0.5mL |
Shipping Condition: | Approved for shipment at Room Temperature or on Wet or Dry Ice |
内容及储存
TrackIt™ Cyan/Orange Loading Buffer is composed of 30% (v/v) glycerol, 60 mM Tris-HCl (pH 7.5), 60 mM EDTA, 0.36% (w/v) XCFF, and 2.4% (w/v) Orange G. TrackIt™ Cyan/Yellow Loading Buffer is composed of 30% (v/v) glycerol, 60 mM Tris-HCl (pH 7.5), 60 mM EDTA, 0.36% (w/v) XCFF, and 3.6% (w/v) Tartrazine. Store at 15°C to 30°C.