描述

MAX Efficiency® DH10B™ Competent Cells are highly efficient E. coli, perfect for most applications. DH10B™ E. coli are available in both electrocompetent and chemically competent formats. The high transformation efficiency makes them ideal for generating cDNA or genomic libraries. High-efficiency transformation is seen with plasmids 150 kb in size. The versatile DH10B™ genotype has the following features:

• lacZ for blue/white screening of recombinant clones
• Elimination of mcrA, mcrBC, mrr, and hsdRMS restriction systems to allow construction of more representative genomic libraries
• A1 mutation to increase plasmid yield and quantity

规格

Competent Cell Type:	Chemically Competent
Species:	E. coli
Format:	Tube(s)
Transformation Efficiency:	> 1x10^9
Bacterial or Yeast Strain:	DH10B™
Product Line:	DH10B™, MAX Efficiency®
Blue-White Screening:	Yes
Cloning Methylated DNA:	Yes
Cloning Unstable DNA:	Not Suitable for cloning unstable DNA
F' Episome (to make ssDNA):	Lacks F' Episome
High Throughput Compatibility:	Not High Throughput-Compatible (Manual)
Improves Plasmid Quality:	Yes
Preparing Unmethylated DNA:	Not Suitable for preparing unmethylated DNA
Product Size:	10 reactions
Propagating ccdB Vectors:	Not for ccdB vector propagation
Reduces Recombination:	Yes
T1 Phage - Resistant (tonA):	No
Shipping Condition:	Dry Ice

内容及储存