描述

The SuperScript® VILO™ cDNA Synthesis Kit provides the high-temperature capability of SuperScript® III Reverse Transcriptase in an optimized format for generating first-strand cDNA for use in real-time quantitative RT-PCR (qRT-PCR). VILO is an acronym for variable input, linear output; and the SuperScript®: VILO™ cDNA Synthesis Kit provides the most consistent results for your RT experiments, with unprecedented linearity across the broadest range of input material.

Using the SuperScript® VILO™ cDNA Synthesis Kit
This formulation provides enhanced cDNA synthesis efficiency and can be used with very low and very high amounts of input RNA (up to 2.5 µg total RNA in a 20 µL reaction), giving a linear response in message abundance as measured by qPCR. The 10X SuperScript® Enzyme Mix includes SuperScript® III RT, RNaseOUT™ Recombinant Ribonuclease Inhibitor, and a proprietary helper protein.

SuperScript® III RT
SuperScript® III RT is an engineered version of M-MLV RT with reduced RNase H activity and increased thermal stability. The enzyme can be used to synthesize cDNA at a temperature range of 42–60°C. Because SuperScript® III RT is not significantly inhibited by ribosomal and transfer RNA, it can be used to synthesize cDNA from total RNA. RNaseOUT™ Recombinant Ribonuclease Inhibitor safeguards against the degradation of target RNA due to ribonuclease contamination.

Optimized buffer
The 5X VILO™ Reaction Mix includes random primers, MgCl ₂, and dNTPs in a buffer formulation that has been optimized for qRT-PCR.

规格

Reverse Transcriptase:	SuperScript® III
Downstream Application:	Real-Time Quantitative PCR (qPCR)
Reaction Format:	SuperMix or Master Mix
Sample Type (General):	RNA
Optimal Reaction Temperature:	42° C
Primer Type:	Random Primers
Product Line:	SuperScript®, VILO™
Product Size:	250 x 20 µL reactions
Shipping Condition:	Dry Ice

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