描述
The ARES™ Alexa Fluor® DNA Labeling Kit provides a versatile, two-step method for labeling DNA with our Alexa Fluor® dyes. In the first step, an amine-modified nucleotide is incorporated into DNA using conventional enzymatic labeling methods. In the second step, the amine-modified DNA is chemically labeled using our proprietary amine-reactive Alexa Fluor® 647 dye. The labeled probes can be used for fluorescence
in situ hybridization (FISH) and microarray techniques. We offer ARES™ Alexa Fluor® DNA Labeling Kits in five fluorescent colors, and each kit provides sufficient reagents for 5 to 10 labeling reactions of 1 to 5 µg DNA each.
ARES™ Alexa Fluor® Labeling Kit Specifications:
• Dye (Ex/Em): Alexa Fluor® 647 (650/670 nm)
• Achieves more uniform, consistent labeling than techniques for enzymatic incorporation of labeled nucleotides
• Typically produces one dye per 12–20 bases
• Optimal for FISH and microarrays More Uniform Labeling With ARES™ Labeling Kits ARES™ Alexa Fluor® Labeling Kits employ a two-step labeling technology—nick translation to enzymatically incorporate an amine-modified nucleotide (aminoallyl dUTP) followed by chemical labeling with Alexa Fluor® dyes. This method achieves uniformity and consistency of labeling that is difficult to obtain with conventional enzymatic incorporation of labeled nucleotides.
We also offer this two-step labeling technology in our
FISH Tag™ DNA and
FISH Tag™ RNA Kits, which provide a complete workflow solution for FISH applications, including all of the reagents for probe synthesis, labeling, purification and an anti-fade reagent to help protect the signal during fluorescence microscopy.
More Options for Nucleic Acid Labeling To review various options for nucleic acid labeling (including DNA and RNA FISH), review
Labeling Oligonucleotides and Nucleic Acids—Section 8.2 in the Molecular Probes® Handbook.
For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.
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