描述
Invitrogen NuPAGE Bis-Tris Midi Gels are precast polyacrylamide gels designed to give optimal separation of a wide range of proteins under denaturing conditions. Adapters are included with these gels that allow the gels to be run on a Bio-Rad Criterion™ Cell. Unlike traditional Tris-glycine gels, NuPAGE Bis-Tris gels have a neutral pH environment that minimizes protein modifications. Use NuPAGE Bis-Tris gels for the preparation of proteins for sequencing, mass spectrometry, and any other techniques where protein integrity is crucial. Also, use NuPAGE gels for optimal results during day-to-day use. NuPAGE Bis-Tris gels come in two sizes and multiple well formats.
Features of NuPAGE Bis-Tris gels:
• Faster run times—get separation of your proteins in as little as 35 minutes
• Longer shelf life—NuPAGE Bis-Tris gels can be stored for at least 12 months at room temperature
• Better protein integrity—optimized sample preparation process preserves your proteins
• Wide ranges of molecular weight separation—select the right gel and running buffer to get the optimal separation of your proteins
Learn more about all of our available NuPAGE Bis-Tris gels > Choose the right NuPAGE Bis-Tris gel for your protein separation Obtain optimal separation of your proteins by choosing the right combination of gel and running buffer. NuPAGE Bis-Tris midi gels come in four polyacrylamide concentrations: 8%, 10%, 12%, and a 4–12% gradient. The gels can be run using NuPAGE MES or NuPAGE MOPS SDS running buffer to obtain different separation ranges.
Choose the right NuPAGE Bis-Tris gel for the size of your samples The gels come in two sizes: mini (10 cm x 10 cm) or midi (8.7 cm x 13.3 cm) and either 1.0 mm (mini and midi gels) or 1.5 mm (mini gel format only) in thickness. NuPAGE Bis-Tris gels also come in multiple well formats.
Fast and convenient to use NuPAGE Bis-Tris gels can be run in as little as 35 minutes when using the NuPAGE MES running buffer. NuPAGE Bis-Tris gels have a shelf life of at least 12 months at 4–25°C. Unlike traditional Tris-glycine gels, NuPAGE Bis-Tris gels have a neutral pH environment that minimizes protein modifications. The gels also provide sharper bands, higher resolution, longer shelf-life, and higher accuracy of results.
NuPAGE Bis-Tris gels are formulated for denaturing gel electrophoresis applications. For optimal sample preparation, use the
NuPAGE LDS Sample Buffer and
NuPAGE Sample Reducing Agent with your samples. Use
NuPAGE Antioxidant in the running buffer to maintain the reduced state of the proteins during the run and allow maximum band sharpness. We recommend using
Novex Sharp Pre-stained Protein Standard; this protein standard contains our widest molecular weight range with 12 protein bands ranging from 3.5–260 kDa. Get fast and sensitive staining of your protein gels with our
SimplyBlue SafeStain.
We also provide
NuPAGE Tris-acetate gels for separating larger proteins. For classic Laemmli-based Tris-Glycine electrophoresis, we offer
Novex Tris-glycine gels.
NuPAGE Bis-Tris Midi gels can be used with the
XCell SureLock Midi Cell gel apparatuses or conveniently with the Bio-Rad Criterion Cell using our
adapters.
For transfer of proteins to a membrane, we recommend using
NuPAGE Transfer buffer. Rapid semi-dry transfer can be done using the
Pierce Power Blotter or rapid dry transfer using the
iBlot 2 Gel Transfer Device. Alternatively, traditional wet transfer can be performed using the Bio-Rad Criterion Cell.
Related links Overview of 1D Protein Electrophoresis Comparison of NuPAGE Tris-Bis vs. traditional Tris-glycine gels 
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