描述

The FreeStyle™ MAX System is a breakthrough technology for rapid and high-yield mammalian protein production. This new mammalian expression technology is available in two convenient formats. FreeStyle™ MAX Reagent is a novel cationic lipid-based reagent designed to transfect FreeStyle™ cells with high efficiency. The FreeStyle™ MAX Expression System combines GIBCO® FreeStyle™ Media, FreeStyle™ MAX Reagent, and either FreeStyle™ CHO-S cells or FreeStyle™ 293 cells. The only equipment required to use the FreeStyle™ MAX System is a CO2 incubator and a platform shaker with shake flasks or a spinner culture.

The FreeStyle™ MAX System provides:
• An easy, rapid protocol for transient transfection of CHO and 293 cells in suspension cultures
• Functional proteins (up to milligram quantities) with all post-translational modifications produced in one week
• Simplified downstream purification of secreted proteins with FreeStyle™ serum-free medium
• Straightforward protocol for production scale-up

The FreeStyle™ MAX System can replace time-consuming stable cell line generation with large-scale functional protein production in one week.

Contents and Storage:
Store FreeStyle™ cells in liquid nitrogen. Store FreeStyle™ MAX Reagent at +4°C. Store GIBCO® FreeStyle™ CHO Expression Medium, FreeStyle™ 293 Expression Medium, and OptiPRO™ SFM at +2-8°C in the dark. Store the pCMV SPORT at -20°C. Stability is guaranteed for 6 months under the recommended storage conditions. Please refer to the manual for additonal storage information.

规格

Cell Line:	FreeStyle™ 293-F Cells
Classification:	Animal Origin-Free, Serum-Free
Culture Type:	Suspension Cell Culture
Delivery Method:	Transfection
Expression:	Mammalian
Expression Mechanism:	Cell-Based Expression
Key Function:	Protein Production
Product Line:	Freestyle™
Product Size:	1 kit