描述
The BenchMark™ Protein Ladder is a molecular weight standard for SDS-PAGE. It consists of 15 recombinant proteins ranging in molecular weight from 10 to 220 kDa and can be used with NuPAGE®,Tris-Glycine, or Tricine gels. The BenchMark™ Protein Ladder offers:
• Extra-intense 20- and 50-kDa orientation bands to ensure proper identification of each protein
• Affinity-purified proteins to provide sharp bands and clear background
• A ready-to-use format for direct application to an SDS-PAGE gel
• Detection with Coomassie® Brilliant Blue R-250, silver, or other protein stain
• Sufficient reagent for 100 mini gels or 50 regular gels using Coomassie® Brilliant Blue R-250 规格
Gel Compatibility: | Bolt® Bis-Tris Plus Gels, Novex® Tricine Gels, Novex® Tris-Glycine Gels, NuPAGE® Bis-Tris Gels, NuPAGE® Tris-Acetate Gels, SDS-PAGE Gels |
Stain Type: | Unstained |
Size Range: | 10 to 220 kDa |
System: | SDS-PAGE, Tris-Glycine |
Product Line: | BenchMark™ |
Product Size: | 2 x 250 µL |
Shipping Condition: | Approved for shipment on Wet or Dry Ice |
内容及储存
Two vials of 250 µl each are provided in loading buffer consisting of 50 mM Tris-HCl (pH 6.8), 2 mM EDTA, 10 mM DTT, 2% (w/v) SDS, 10% (w/v) glycerol, 0.01% (w/v) bromophenol blue. Store at -20°C. Avoid repeated freezing and thawing.