描述
DH10B™ T1 Phage-Resistant E. colihave all the benefits of the DH10B™ genotype, with the addition of the tonA genotype which confers resistance to T1 and T5 phage infection. The inclusion of the tonA genotype protects your valuable samples and minimizes the possibility of downtime in your lab due to phage contamination. The DH10B™-T1 R genotype has the following features:
• tonA genotype to confer resistance to T1 and T5 phage
• Elimination of mcrA, mcrBC, mrr, and hsdRMS restriction systems to allow construction of more representative genomic libraries (1,2)
• The endA1 mutation to increase plasmid yield and quantity
ZΔM15 for blue/white screening of recombinant clones
DH10B™-T1 R E. coliare available in both electrocompetent and chemically competent formats. The high transformation efficiency makes them ideal for generating cDNA or genomic libraries. High efficiency transformation is seen with plasmids 150 kb in size (3). 规格
Competent Cell Type: | Chemically Competent |
Species: | E. coli |
Format: | One Shot |
Transformation Efficiency: | > 1x10^9 |
Bacterial or Yeast Strain: | DH10B™ |
Product Line: | DH10B™, MAX Efficiency®, One Shot® |
Blue-White Screening: | Yes |
Cloning Methylated DNA: | Yes |
Cloning Unstable DNA: | Not Suitable for cloning unstable DNA |
F' Episome (to make ssDNA): | Lacks F' Episome |
High Throughput Compatibility: | Not High Throughput-Compatible (Manual) |
Improves Plasmid Quality: | Yes |
Preparing Unmethylated DNA: | Not Suitable for preparing unmethylated DNA |
Product Size: | 20 reactions |
Propagating ccdB Vectors: | Not for ccdB vector propagation |
Reduces Recombination: | Yes |
T1 Phage - Resistant (tonA): | Yes |
内容及储存
Store in ultra-cold freezer (-68 to -85°C).