描述
Novex Tris-Glycine Plus Midi Protein gels are improved polyacrylamide gels based on traditional Laemmli protein electrophoresis technology, allowing the use of Laemmli sample and running buffers. The gels offer reproducible separation of a wide range of proteins into well-resolved bands. Novex Tris-Glycine Plus Midi gels are a 1.0 mm thick, wider format (8 cm x 13 cm) gel for higher throughput electrophoresis of protein samples.
Features of Novex Tris-Glycine Plus gels include:
Improved shelf life—store gels for up to 12 months at 4°C
Flexibility—use for native and denaturing protein assays
Fast run conditions—quickly separate your proteins using constant voltage in less than 60 minutes
Choose the right gel format for your experiments Novex Tris-Glycine Plus Midi gels come in fixed concentrations of 10% and 12%, as well as gradient concentrations of 4–12%, 4–20%, and 8-16%. Select from our many well formats, including 12+2-, 20-, and 26-well.
Run your proteins in native or denatured form Novex Tris-Glycine Plus gels do not contain SDS and so can be used to separate proteins in native or denatured form. For denatured proteins, we recommend using
Novex Tris-Glycine SDS Sample Buffer and
Novex Tris-Glycine SDS Running Buffer. For native proteins, we recommend using
Novex Tris-Glycine Native Sample Buffer and
Novex Tris-Glycine Native Running Buffer. The gels can be run using our
XCell4 SureLock Midi-Cell or conveniently with the Bio-Rad Criterion Cell using
our adapters.
For transfer of proteins to a membrane, we recommend using the
Novex Tris-Glycine Transfer Buffer. Rapid semi-dry transfer can be done using the
Pierce Power Blotter or rapid dry transfer using the
iBlot 2 Gel Transfer Device. Alternatively, traditional wet transfer can be performed using the Bio-Rad Criterion™ Cell.
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