描述
The AccuPrime™ Taq DNA Polymerase System provides reagents for amplification of nucleic acid templates with an antibody-mediated, hot-start protocol for improved PCR specificity over other hot-start DNA polymerases (see figure). Platinum® anti- Taq DNA polymerase antibodies inhibit polymerase activity, providing an automatic hot-start, while a thermostable accessory protein enhances specific primer-template hybridization during every cycle of PCR. This combination improves the fidelity of Taq DNA Polymerase by two-fold and is ideal for high-throughput screening and multiplex PCR. AccuPrime™ Taq DNA Polymerase broadens primer annealing temperatures, giving you optimal performance between 55°C and 65°C. Advantages of AccuPrime™ Taq DNA Polymerase:
• Specificity—control mispriming at every cycle to get single-band PCR products
• Ease of use—minimize reaction optimization and primer set redesign
• Convenience—conveniently assemble reactions at room temperature
AccuPrime™ buffers
The 10X AccuPrime™ buffers contain thermostable AccuPrime™ protein, Mg ++, and deoxyribonucleotide triphosphates at concentrations sufficient to allow amplification during PCR. Two individual buffer systems (10X AccuPrime™ PCR Buffer I and II) are provided for amplification of specific types of templates. 10X AccuPrime™ PCR Buffer I is designed for small genomic DNA amplicons (≤200bp), cDNA, or plasmids. 10X AccuPrime™ PCR Buffer II is designed for genomic DNA (200 bp–4 kb).
Applications
AccuPrime™ Taq DNA Polymerase can be used for a wide variety of applications, including multiplex PCR, TOPO TA Cloning®, and allele-specific amplifications. 规格
| Polymerase: | AccuPrime™ Taq DNA Polymerase |
| Hot Start: | Built-In Hot Start |
| Fidelity (vs. Taq): | 2 X |
| GC-Rich PCR Performance: | Low |
| Reaction Format: | Separate Components |
| Product Overhang: | 3'-A |
| Reaction Speed: | Standard |
| Final Product Size(s): | 5 kb or less |
| Exonuclease Activity: | 5' - 3' |
| Product Line: | AccuPrime™ |
| Product Size: | 1,000 reactions |
| Shipping Condition: | Approved for shipment on Wet or Dry Ice |
内容及储存
AccuPrime™ Taq DNA Polymerase is provided in a 1000 reaction kit. Each kit includes:
• AccuPrime™ Taq DNA Polymerase (500 µL, at 2U/µL)
• 10X AccuPrime™ PCR Buffer I* (2 × 1.25mL)
• 10X AccuPrime™ PCR Buffer II* (2 × 1.25mL)
• 50 mM Magnesium Chloride (500 µL)
*10XAccuPrime™ PCR Buffer I is designed for small genomic DNA amplicons (≤200 bp), cDNA, or plasmids. 10X AccuPrime™ PCR Buffer II is designed for genomic DNA (200 bp–4 kb).
10X AccuPrime™ PCR Buffer contains 200 mM Tris-HCl (pH 8.4), 500 mM KCl, 15 mM MgCl 2, 2 mM dGTP, 2 mM dATP, 2 mM dTTP, 2 mM dCTP, thermostable AccuPrime™ protein, 10% glycerol.
Store at -20°C.
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