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AccuPrime™ Taq DNA Polymerase System 200 reactions

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号:12339016

牌:Invitrogen

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描述

AccuPrime™ Taq DNA Polymerase provides reagents for amplification of nucleic acid templates with antibody-mediated hot-start for improved PCR specificity over other hot-start DNA polymerases (Figure 1). Platinum® anti- Taq DNA polymerase antibodies inhibit polymerase activity, providing an automatic hot-start, while a thermostable accessory protein enhances specific primer-template hybridization during every cycle of PCR. This combination improves the fidelity of Taq DNA Polymerase by two-fold and is ideal for high-throughput screening and multiplex PCR. AccuPrime™ Taq DNA Polymerase broadens primer annealing temperatures, giving you optimal performance between 55°C and 65°C.

Specificity – Control mispriming at every cycle to get single-band PCR products
Ease of Use – Minimize reaction optimization and primer set redesign
Applications – Multiplex PCR, TOPO TA Cloning®, allele-specific amplifications
Convenience – Conveniently assemble reactions at room temperature

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

规格

Polymerase: AccuPrime™ Taq DNA Polymerase
Hot Start: Built-In Hot Start
Fidelity (vs. Taq): 2 X
GC-Rich PCR Performance: Low
Reaction Format: Separate Components
Product Overhang: 3'-A
Reaction Speed: Standard
Final Product Size(s): 5 kb or less
Exonuclease Activity: 5' - 3'
Product Line: AccuPrime™
Product Size: 200 reactions
Shipping Condition: Approved for shipment on Wet or Dry Ice

内容及储存

AccuPrime™ Taq DNA Polymerase is provided in 200- or 1,000-reaction kits. Each kit includes AccuPrime™ Taq DNA Polymerase, and 10X AccuPrime™ PCR Buffer I & II, which include dNTPs and 50 mM MgCl 2. AccuPrime™ SuperMix I is supplied at 2X concentration [40 mM Tris-HCl (pH 8.4), 100 mM KCl, 3 mM MgCl 2, 400 µM dGTP, 400 µM dATP, 400 µM dTTP, 400 µM dCTP, AccuPrime™ Taq DNA Polymerase, thermostable AccuPrime™ protein, and stabilizers]. AccuPrime™ SuperMix II is supplied at 2X concentration [40 mM Tris-HCl (pH 8.4), 100 mM KCl, 3 mM MgCl 2, 400 µM dGTP, 400 µM dATP, 400 µM dTTP, 400 µM dCTP, AccuPrime™ Taq DNA Polymerase, thermostable AccuPrime™ protein, and stabilizers].
Store at -20° C. Guaranteed stable for 6 months when properly stored.

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