描述

The pENTR™ 5’-TOPO® TA Cloning® Kit combines Invitrogen’s TOPO® Cloning and MultiSite Gateway® Technologies to facilitate five-minute, one-step cloning of a Taq polymerase-amplified PCR product encoding a eukaryotic promoter of interest into a MultiSite Gateway® entry vector with ≥ 85% efficiency. Once cloned, your eukaryotic promoter may be transferred from the pENTR™ 5'-TOPO® vector to a suitable MultiSite Gateway® destination vector in a MultiSite Gateway® LR recombination reaction with other suitable entry clone(s) to generate an expression construct.

This kit can be used in combination with ViraPower™ HiPerform Promoterless Lentiviral Gateway® Vector Kit, or MultiSite Gateway® Three-Fragment Vector Construction Kit.

Advantages
• Choose the best promoter for your experiments and cell line by efficiently expressing your gene of interest from multiple promoter constructs
• When using in conjunction with ViraPower™ HiPerform™ Promoterless Lentiviral vector, you can efficiently transduce and express your gene of interest driven by the promoter of choice in both dividing and non-dividing mammalian cells


Key Features
• Rapid PCR cloning with TOPO® cloning technology for cloning the 5’ element
• attR1-attR4 sites for compatibility with MultiSite Gateway® LR reaction for increased flexibility

Kit Includes
• pENTR™ 5´-TOPO® TA Cloning® Kit
• One Shot® TOP10 Chemically Competent E. coli (C4040-03)

Related SKUs
• ViraPower™ HiPerform™ Promoterless Lentiviral Gateway® Expression System (A111451)
• ViraPower™ HiPerform™ Promoterless Gateway® Vector Kit (A11146)
• MultiSite Gateway® Three-Fragment Vector Construction Kit (12537-023)

For research use only. Not intended for any therapeutic or diagnostic use.

规格

Vector Type:	TOPO-TA Cloning Vectors, pENTR
Cloning Method:	TOPO®-TA
Product Line:	Gateway®, One Shot®, TA Cloning®, TOPO®, pENTR™
Product Size:	20 reactions

内容及储存