描述

The Fluo-4 Direct™ Calcium Assay Kit was formulated to provide a homogeneous fluorescent calcium assay that:
1. Easily loads into cells
2. Achieves a large assay window and
3. Suppresses background fluorescence generated from the calcium indicator in complete media with little-to-no impact on the specific cellular fluorescence

This advanced formulation allows the assay to be run in a simple “addition only" format in the presence of serum-containing media. Fluo-4 Direct™ is the third addition to the Fluo-4 family of calcium detection reagents. Fluo-4 AM and Fluo-4 NW both require media removal before assay detection, but Fluo-4 NW adds convenience by including the PowerLoad™ reagent for ease in cell loading. The Fluo-4 Direct™ Calcium Assay Kit is similar to Fluo-4 NW in that it is formulated with PowerLoad™ for ease in cell loading, but unique in that it can be used in the presence of complete culture media and will efficiently suppress background fluorescence without sacrificing the specific cellular fluorescence generated in the assay.

规格

Indicator Type:	Calcium Indicator
Indicator Properties:	Fluorescent Dye-Based
For Use With (Equipment):	Confocal Microscope, Fluorescence Microscope, Fluorescent Imager, HTS Reader, High Content Instrument, Microplate Reader
Cell Permeability:	Cell-Permeant
Color:	Green
Detection Method:	Fluorescent
Emission Class:	Visible
Excitation Class:	Visible
Excitation⁄Emission (nm):	488⁄530
Form:	Powder
Product Line:	Molecular Probes®
Product Size:	1 x 100mL
Sub-Cellular Localization:	Cytoplasm, Nucleus, Organelles
Volume (Metric):	100mL
Shipping Condition:	Room Temperature

内容及储存