描述

AccuPrime™ GC-Rich DNA Polymerase is designed to provide high-yield, high-specificity amplification of difficult-to-amplify templates, such as those with >65% GC content. The kit offers a choice of buffers for amplifying genomic DNA targets (Buffer A) or non-GC-rich cDNA, plasmid, and lambda-based targets (Buffer B). Benefits of using AccuPrime™ GC-Rich DNA Polymerase:

• Yield—high yields for targets up to 5 kb in length
• Specificity—AccuPrime™ accessory proteins for improved PCR specificity
• Sensitivity—sensitivity down to 5 ng of template DNA

Robust and specific amplifications
The AccuPrime™ buffers contain thermostable proteins that enhance primer-template hybridization during PCR, increasing the specificity of the reaction. The polymerase itself, from the archaebacterium Pyrolobus fumarius, has a five-fold better processivity than Taq DNA polymerase, and remains active even after 4 hours at 95°C.

Unit definition
One unit of AccuPrime™ GC-Rich DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of dNTPs into acid-insoluble material in 30 min at 74°C.

规格

Polymerase:	AccuPrime™ GC-Rich DNA Polymerase
Hot Start:	Built-In Hot Start
Fidelity (vs. Taq):	2 X
GC-Rich PCR Performance:	High
Reaction Format:	Separate Components
Product Overhang:	Blunt
Reaction Speed:	Standard
Final Product Size(s):	5 kb or less
Exonuclease Activity:	3' - 5'
Product Line:	AccuPrime™
Product Size:	1,000 reactions
Shipping Condition:	Approved for shipment on Wet or Dry Ice

内容及储存