描述
AccuPrime™ GC-Rich DNA 聚合酶能高产量、高特异性地扩增难于扩增的模板，比如 GC 含量 > 65% 的模板（图 1）。 AccuPrime™ GC-Rich 提供：
· 经过优化，可用于高 GC 含量和难扩增靶标的酶
·可提高 PCR 特异性的 AccuPrime™ 辅助蛋白质
·对于长达 5 kb 的靶标可获得高产量
·可灵敏检测低至 5 ng 的模板 DNA
·可选择不同缓冲液，用于扩增基因组 DNA 靶标（缓冲液 A）或 cDNA、质粒和 lambda 靶标（缓冲液 B）
单位定义：
一个单位的 AccuPrime™ GC-Rich DNA 聚合酶是在 74°C，在 30 分钟内向不溶于酸的材料掺入 10 纳摩尔 dNTP 所需的酶量。 规格
| Polymerase: | AccuPrime™ GC-Rich DNA Polymerase |
| Hot Start: | Built-In Hot Start |
| Fidelity (vs. Taq): | 2 X |
| GC-Rich PCR Performance: | High |
| Reaction Format: | Separate Components |
| Product Overhang: | Blunt |
| Reaction Speed: | Standard |
| Final Product Size(s): | 5 kb or less |
| Exonuclease Activity: | 3' - 5' |
| Product Line: | AccuPrime™ |
| Product Size: | 200 reactions |
| Shipping Condition: | Approved for shipment on Wet or Dry Ice |
内容及储存
AccuPrime™ GC-Rich DNA Polymerase is supplied with two 5X reaction buffers and a separate vial of 50 mM MgSO 4 . Both buffers contain dNTPs, MgSO 4 , and AccuPrime™ accessory proteins. Reaction buffer A is for genomic targets. Reaction buffer B is for use with cDNA, plasmid, or lambda targets. Store at -20°C. Guaranteed stable for 6 months when properly stored.
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