描述

The Galacto-Star™ One-Step β-galactosidase Reporter Gene Assay System chemiluminescent reporter gene assay system enables rapid, sensitive detection of β-galactosidase in mammalian cell lysates.

• Glow assay kinetics provides a "window" during which measurements may be performed, facilitating HTS applications where assay automation is used.
• Can be used with either mammalian or yeast model systems, providing flexibility in choice of model systems.
• Wide dynamic range of β-galactosidase assay lets the user measure enzyme level accurately from femtogram to nanogram range.
• Assay sensitivity is 100- to 1,000-fold better than either the isotopic⁄non-isotopic assays for chloramphenicol acetyl transferase (CAT) or the colorimetric⁄fluorescent assays for β-galactosidase, providing greater sensitivity than competing assays.
• Highly sensitive assay with a wide dynamic range permits detection of high and low levels of reporter without performing numerous sample dilutions.
• Non-radioactive reporter gene assay kit eliminates concerns over use of radioisotopes.
• Assay can be completed in about one hour, providing fast assay turnaround.

Sensitive
The chemiluminescent assay exhibits over three orders of magnitude greater sensitivity than colorimetric β-galactosidase assays and can be completed in a fraction of the time required for assays for chloramphenicol acetyltransferase (CAT) and ELISA-based reporter assays.

Versatile
The Galacto-Star™ system includes Galacton-Star® chemiluminescent substrate for β-galactosidase and is suited for measurement in both luminometers and scintillation counters. Automatic injection is not required.

Long-Lasting Signal
Light emission from the Galacto-Star™ assay reaches maximum in 60-90 minutes and remains constant for nearly one hour. Cell lysate is mixed with reaction buffer containing Galacton-Star® substrate and Sapphire-II™ luminescence enhancer. After a 60-90 minute incubation at room temperature, the signal is measured in a luminometer or scintillation counter.

For Research Use Only. Not for use in diagnostics procedures.

规格

Assay Type:	β-Gal (lacZ) Assay
Compatible Cells:	Mammalian Cells
Detection Method:	Chemiluminescent
For Use With (Equipment):	Luminometer (Microplate)
Form:	Liquid
Format:	96-well plate
High Throughput Compatibility:	High Throughput-Compatible
Product Line:	Applied Biosystems™, Galacto-Star™, NovaBright™
Product Size:	1,800 assays
Readout:	End Point
Sample Type (Specific):	Cell Cultures
Substrate:	Galacton-Star®
Substrate Properties:	Chemical Substrate
Substrate Type:	Beta-Gal Substrate
Target Enzyme:	Beta-Galactosidase
Technique:	Enhanced Chemiluminescence

内容及储存