描述
The Galacto-Star™ One-Step β-Galactosidase Reporter Gene Assay System chemiluminescent reporter gene assay system enables rapid, sensitive detection of β-galactosidase in mammalian cell lysates.
• Glow assay kinetics provides a "window" during which measurements may be performed, facilitating HTS applications where assay automation is used.
• Can be used with either mammalian or yeast model systems, providing flexibility in choice of model systems.
• Wide dynamic range of β-galactosidase assay lets the user measure enzyme level accurately from femtogram to nanogram range.
• Assay sensitivity is 100- to 1,000-fold better than either the isotopic⁄non-isotopic assays for chloramphenicol acetyl transferase (CAT) or the colorimetric⁄fluorescent assays for β-galactosidase, providing greater sensitivity than competing assays.
• Highly sensitive assay with a wide dynamic range permits detection of high and low levels of reporter without performing numerous sample dilutions.
• Non-radioactive reporter gene assay kit eliminates concerns over use of radioisotopes.
• Assay can be completed in about one hour, providing fast assay turnaround.
Sensitive
The chemiluminescent assay exhibits over three orders of magnitude greater sensitivity than colorimetric β-galactosidase assays and can be completed in a fraction of the time required for assays for chloramphenicol acetyltransferase (CAT) and ELISA-based reporter assays.
Versatile
The Galacto-Star™ system includes Galacton-Star® chemiluminescent substrate for β-galactosidase and is suited for measurement in both luminometers and scintillation counters. Automatic injection is not required.
Long-Lasting Signal
Light emission from the Galacto-Star™ assay reaches maximum in 60-90 minutes and remains constant for nearly one hour. Cell lysate is mixed with reaction buffer containing Galacton-Star® substrate and Sapphire-II™ luminescence enhancer. After a 60-90 minute incubation at room temperature, the signal is measured in a luminometer or scintillation counter.
For Research Use Only. Not for use in diagnostics procedures. 规格
Assay Type: | β-Gal (lacZ) Assay |
Compatible Cells: | Mammalian Cells |
Detection Method: | Chemiluminescent |
For Use With (Equipment): | Luminometer (Microplate) |
Form: | Liquid |
Format: | 96-well plate |
High Throughput Compatibility: | High Throughput-Compatible |
Product Line: | Applied Biosystems™, Galacto-Star™, NovaBright™ |
Product Size: | 600 assays |
Readout: | End Point |
Sample Type (Specific): | Cell Cultures |
Substrate: | Galacton-Star® |
Substrate Properties: | Chemical Substrate |
Substrate Type: | Beta-Gal Substrate |
Target Enzyme: | Beta-Galactosidase |
Technique: | Enhanced Chemiluminescence |
内容及储存
This kit contains the lysis solution, substrate, enhancer, and required buffer. Store the product at 4°C.