描述
CaptAvidin™ biotin-binding protein conjugated to agarose is designed for use in affinity chromatography. CaptAvidin™ biotin-binding protein contains a nitrated tyrosine in its biotin-binding site. This chemical modification permits dissociation of the avidin-biotin complex under milder conditions than the traditional avidin-biotin complex. At pH 4.0, CaptAvidin™ biotin-binding protein binds biotin tightly with a K a of ~10 9M -1. This association can then be reversed at pH 10, allowing complete dissociation of the avidin-biotin complex. This is in contrast to the harsh conditions (8 M guanidine hydrochloride, pH 1.5) needed to dissociate the avidin-biotin complex. 规格
Label or Dye: | Agarose |
Product Size: | 5mL |
Shipping Condition: | Wet Ice |
内容及储存
Store in refrigerator (2–8°C).