描述
MultiScribe™ Reverse Transcriptase is a recombinant moloney murine leukemia virus (rMoMuLV) reverse transcriptase that has been optimized for TaqMan-based assays. MultiScribe™ Reverse Transcriptase is similar to MuLV reverse transcriptase but differs in its recommended usage. MultiScribe™ Reverse Transcriptase is a recombinant RNA–dependent DNA polymerase that uses single-stranded RNA as a template in the presence of a primer to synthesize a complementary DNA (cDNA) strand. 5,000 U is supplied in 100 µL vials (50 U/µL). Advantages of MultiScribe™ Reverse Transcriptase:
• Compatible with one-step and two-step RT-PCR reactions, which provides flexibility in assay setup
• Individual component design provides versatility in assay setup 规格
Optimal Reaction Temperature: | 42-52° C |
Final Product Size(s): | 5 kb or less |
Reverse Transcriptase: | MultiScribe™ Reverse Transcriptase |
Ribonuclease H Activity: | Reduced |
Concentration: | 50 units⁄µl |
Enzyme Function: | RNA dependent DNA Polymerase |
Product Line: | Applied Biosystems™, MultiScribe™ |
Product Size: | 100 µL |
Sensitivity: | High |
Shipping Condition: | Dry Ice |
Template: | ssRNA |
内容及储存
Contents: MultiScribe™ Reverse Transcriptase (100 µL at 50U/µL). Store for up to one year at -20°C.