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Interleukin-1 beta (IL-1β) is a non-secreted proinflammatory cytokine produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. Both IL-1α and IL-1β binds to the same receptor and has similar but not identical biological properties. Recombinant equine interleukin-1 beta is a 17kDa protein containing 153 amino acid residues and it shares 63%~70% a.a. sequence identity with canine, cotton rat, porcine, feline, human, mouse, rat, and rhesus IL1β.
This material is offered by Sangon Biotech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Purity | >95% by SDS-PAGE and HPLC analyses. |
| Source | Escherichia coli. |
| Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 20 pg/ml, corresponding to aspecific activity of>5.0×107 IU/mg. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 1×PBS, pH 7.4, 0.1% Tween-80. |
| Endotoxin | Less than 1 EU/μg of rEqIL-1β as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 ?C. Further dilutions should be made in appropriate buffered solutions. |
| AA Sequence | AAMHSVNCRL RDIYHKSLVL SGACELQAVH LNGENTNQQV VFCMSFVQGE EETDKIPVAL GLKEKNLYLS CGMKDGKPTL QLETVDPNTY PKRKMEKRFV FNKMEIKGNV EFESAMYPNW YISTSQAEKS PVFLGNTRGG RDITDFIMEI TSA |
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