Western Blot is a powerful tool for protein analysis. This kit provides rapid chromogenic detection of mouse, rat or rabbit primary antibodies. The kit utilizes horseradish peroxidase conjugated anti-IgG antibody and the substrate 3, 3’-diaminobenzidine (DAB) to produce a brown precipitate. All reagents together with PVDF/NC membrane are sufficient to analysize 50~100 samples total. The highly purified secondary antibody conjugates and matching reagents provide good sensitivity, minimize species cross-reactivity.Applications: It can analyze 50~100 samples for Western Blot.
Rat HRP Conjugated Secondary Antibody;NC(10 x 10 cm);Transfer Mixture ;Ponseau S 10 x Buffer;Block Reagent;20 x PBS;20 x TBS;Tween-20;DAB;10 x DAB Development Solution;Hydrogen Peroxide( H2O2 );Heat-Sealable Bag
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